Abstract : 1H NMR spectra from plasma samples which had been snap-frozen and stored for 48 h at -80°C were compared with spectra from fresh plasma samples. A slight decrease in some lipids resonances at ? 0.8-0.9 (methyl region), ? 1.2-1.3 and 1.57 (methylene region) and ? 5.3-5.4 (methine region) was observed in spectra from snap-frozen plasma. The broadening of lipoprotein resonances has previously been described in human plasma stored at -20 °C for 5 days (Bell, Sadler et al. 1987; Bell, Brown et al. 1988). The sample stability was observed for three freeze thaw processes and only cholesterol ester had shown significant decrease in concentration out of 786 metabolites (Zivkovic et al, eff3ects of sample handling and storage on quantitative lipid analysis, 2009).
Cite : Srivastava, S., & Roy, R. (2022). Resonance And Effect Of Physical Variables In Serum Metabolomics (1st ed., p. 15). Global Research Foundation. https://doi.org/10.52458/9789391842741.2022.eb.grf.asu.ch-03
