Abstract : TLC is a tool for analyzing mixtures, by separating the mixture's constituent chemicals. TLC has the potential to identify compounds, establish their identities, and ascertain the purity of a chemical. It may also be used to trail the development of a reaction by keeping an eye on the arrival of a product or the disappearance of a reactant. TLC is a quick and sensitive method that can examine microgram (0.000001 g) amounts in a short amount of time (about 5-10 minutes). Three phases make up TLC: spotting, development, and visualizing. To create an extremely diluted (approximately 1%) solution, the sample to be studied is first dissolved in a volatile (easily evaporated) solvent.
